Love it or hate it (hate it) the default glass look iPhone gives to home screen icons is here to stay. There’s no way to disable it for Home Clip icons (for web apps) and it’s not recommended to do so with native apps. Fine, whatever. I’ll deal with it.
Still, it’s kinda hard to design with this “improvement” in mind. You have to save your image, load the app on your iPhone or in the simulator, add it to the home screen, stare at it, then remove it when you’re done. Lame.
This might make the process easier - I made a simple Photoshop file with layers which attempt to mimic as closely as possible the gloss effect that gets slapped on. Throw this layer group over your design and get a real-time preview. Yay.
iPhone icon gloss layers (Photoshop CS3, should work in most versions)
I hope this helps in a small way.